拮抗菌株XZQ-20的鉴定及对多种植物病原细菌的拮抗活性分析

黄华毅¹, 于地美², 黄咏槐¹, 黄焕华¹, 田呈明³

1.Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization/Guangdong Academy of Forestry;2.Patent Examination Cooperation Center of The Patent Office;3.The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University

摘要:

利用拮抗微生物不仅能有效防治植物病原细菌，而且对环境友好。该研究中，拮抗菌株 XZQ- 20 分离自健康杨树根际土壤，基于形态学特征、生理生化特性和 16S rDNA 序列分析将该菌株鉴定为深层类芽孢杆菌（Paenibacillus profundus），XZQ-20 具有蛋白酶活性，其对 4 种植物病原细菌欧美杨细菌性溃疡病菌（Lonsdalea quercina）、丁香假单胞菌（Pseudomonas syringae）、软腐欧文氏菌（Erwinia carotovora）和核桃黄极毛杆菌（Xanthomonas juglandis）均具有较强的拮抗活性，抑菌圈大小分别为（11.75±1.06）mm、（13.3±2.26）mm、（16.75±0.07）mm 和（7.55±1.06）mm。此外，对拮抗菌株 XZQ-20 的无菌培养滤液的拮抗活性进行测定，发现其无菌培养滤液也表现出了较强的拮抗活性，对 4 种植物病原细菌的抑菌圈直径分别达到（8.18±0.75）mm、（7.63±0.88）mm、（8.03±0.93）mm 和（8.60±0.53）mm。研究结果表明拮抗菌株 XZQ-20 可用作为潜在的高效防治植物病原细菌的生防制剂。

关键词: 植物病原细菌；拮抗活性；鉴定；深层类芽孢杆菌；无菌培养滤液

DOI：

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基金项目：无

Identification and Antagonistic Activity of Strain XZQ-20 Against Plant Pathogenic Bacteria of Various Taxonomic Groups

Huang Huayi¹, Yu Dimei², Huang Yonghuai¹, Huang Huanhua¹, Tian Chengming³

Abstract:

The use of antagonistic microorganisms may effectively control plant pathogenic bacteria in a manner that is also safer for the environment. In this study, a strain XZQ-20 was isolated from the rhizosphere soil of healthy poplar plant, and was identified as Paenibacillus profundus according to its morphological and physiological characteristics, as well as a phylo投注app analysis of partial 16S rDNA sequence. Strain XZQ- 20 has activity of protease and showed good antagonistic activities against four plant pathogenic bacteria (Lonsdalea quercina, Pseudomonas syringae, Erwinia carotovora and Xanthomonas juglandis) with inhibition zones of (11.75±1.06) mm, (13.3±2.26) mm, (16.75±0.07) mm, and (7.55±1.06) mm, respectively. The sterile culture filtrate of the strain XZQ-20 was also found to be greatly antagonistic against the four plant pathogenic bacteria with inhibition zones of (8.18±0.75) mm, (7.63±0.88) mm, (8.03±0.93) mm, and (8.60±0.53) mm,respectively. These results suggest that strain XZQ-20 can be used as a potential biocontrol agent against plant pathogenic bacteria.

Key words: plant pathogenic bacteria; antagonistic activity; identification; Paenibacillus profundus; sterile culture filtrate

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拮抗菌株XZQ-20的鉴定及对多种植物病原细菌的拮抗 活性分析

拮抗菌株XZQ-20的鉴定及对多种植物病原细菌的拮抗活性分析