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陈颖乐1, 曾雷1, 王彩云2, 王颂2, 徐巧林2, 余玉娟2, 黄文妍2
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1.广东省森林培育与保护利用重点实验室/投注app;2.1
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摘要: |
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以香花崖豆藤（Millettia dielsiana）茎段为外植体，通过双因素试验和正交试验分别对茎段腋
芽增殖、生根的影响因素进行筛选，建立了香花崖豆藤的快速繁殖体系。结果表明：香花崖豆藤茎段腋
芽增殖培养最佳培养基为 NAA 0.1 mg · L-1 + 6-BA 0.8 mg · L-1 MS；最适生根培养基为 IBA 0.5 mg · L-1、
NAA 0.6 mg · L-1、GGR6 mg · L-1，生根率为 86.0%，形成了可以进行产业化的香花崖豆藤组织培养技术。
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关键词:
香花崖豆藤；腋芽；组织培养；生根率
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DOI：
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分类号：
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Tissue Culture Technology of Millettia dielsiana |
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chen ying le1, zeng lei1, 12, 12, 12, 12, 12
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1.Guangdong Academy of Forestry;2.1
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Abstract: |
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Using stem segment of Millettia dielsiana as explants, the influence factors of axillary bud
proliferation and rooting in stem segment were screened by two-factor test and orthogonal test, and the rapid
propagation system of stem segment was established. The results showed that NAA 0.1 mg · L-1 + 6-BA 0.8 mg · L- 1
MS was the optimal medium for axillary bud proliferation culture of stem segment. The optimal rooting medium
was IBA 0.5 mg · L1
, NAA 0.6 mg · L-1 and GGR6 mg · L-1, and the rooting rate was 86.0%. The technology of
tissue culture of M. dielsiana has been developed.
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Key words:
Millettia dielsiana；axillary bud；tissue culture；rooting rate
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